Then, cDNA was used in nested PCR performed with a first round of the following PCR primers forward primer 5 primer Dux4 F_Ex1 Nested1 CTCAGCGAGGAAGAATACC and 5 reverse primer Adapter Nested1 GAATCGAGCACCAGTTACGCATG a second round of PCR was performed with forwards primer forward primer, 5 CTCAGCGAGGAAGAATACC 3, and reverse primer, 5 GAATCGAGCACCAGTTACGCATG 3 is nolvafien the same as nolvadex
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