20, 43, 44 For p21 and BAK promoter assays, PCR primers were previously identified and 2 ВµL out of the 20 ВµL of extracted DNA was used in 50 cycles of amplification in 3 steps 95 C for 15 seconds, 55 C for 30 seconds, and 68 C for 45 seconds order cialis What mistakes have you learned on your journey